Using combination of diatomite adsorption and non-covalent modification by poly(ethylene glycol) 4000 (PEG), a novel method of immobilizing Candida antarctica lipase (CAL) to give a high catalytic activity for transesterification reaction in anhydrous organic media was developed. The transesterification activity of the immobilized lipase was assayed in the transesterification reaction between 1,2,3-oleic acid and methanol. The optimum immobilization conditions of the CAL were found as follows: diatomite : l...