A novel method for the preparation of the glucose biosensor was described.Thionine was electropolymerized on the glassy carbon electrode to form a negatively charged surface by cyclic voltammetry.Nano-Au/glucose oxindase(GOX) were immobilized on the electrode modified by poly(thionine) via electrostatic adsorption respectively,then the modified electrode was combined with poly-thionine to prepare the glucose biosensor.The biosensor shows fast response,high sensitivity and stability.The linear response of th...