Degenerate primers were designed based on the conserved sequences of the AKT1 genes from other plants for investigating molecular mechanisms of K+/Na+ selective absorption in P.tenuiflora.Total RNA was extracted from the roots of Puccinellia tenuiflora to obtain an AKT1 gene fragment by reverse transcription polymerase chain reaction(RT-PCR).The sequencing result shows that the AKT1 gene fragment(about 1019 bp) from Puccinellia tenuiflora encodes 339 amino acids.Homology comparison show that the AKT1 gene f...
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