Bilirubin oxidase (EC.1.3.3.5) produced by Myrothecium verrucaria shows a promising potential in the application of environmental protection.In order to increase the production of bilirubin oxidase by M.verrucaria,we investigated the effects of carbon,nitrogen,metal ions and inducers using the single-factor and orthogonal design methods.The results show that glucose and soya peptone are the best carbon and nitrogen sources,respectively.A higher enzyme activity was achieved with the addition of 2 mmol /L Cu2...