Twenty-nine cultivars in the genus Chaenomeles were used as materials to establish the AFLP(Amplified Fragment Length Polymorphism)reaction system for studying the relationship and genetic diversity in flowering quince resources.The AFLP analysis system was established by detected the results of several reactions such as DNA extraction,enzymes restriction,pre-amplification and selective amplification;the main factors which affected AFLP analysis were analyzed.Eight pairs of primers,E-AAG+M-CAA,E-ACA+M-CAA,E...
AFLP(Amplified fragment length polymorphism)即扩增片段长度多态性[1]是一项新的DNA指纹技术,结合了RFLP和PCR技术的特点,具有RFLP技术的可靠性和PCR技术的高效性。该技术采用同位素标记放射自显影方法,稳定性好,条带也清晰,已被广泛用于检测遗传多样性、指纹图谱和品种鉴定