The crossing fruits were used as explants in this paper to study the embryo culture of sweet cherry.The results indicated that the proper medium for embryo culture of sweet cherry was MS+BA 1 mg/L+IAA 1 mg/L+sucrose 30 g/L+agar 6 g/L and for proliferation was MS+BA 0.5 mg/L+sucrose 30 g/L+agar 6 g/L;The appropriate medium for roots induction was 1/2MS+IBA 0.4 mg/L+sucrose 30 g/L+agar 6 g/L.