Isaria farinosa is one of the most common species of entomopathogenic fungi,and has been used as biocontrol agents against some pests.The structural gene of subtilisin-like protease,with 1650 bp,was cloned from Isaria farinosa,and its upstream sequence was amplified by using genome walking.The amplified structural gene has a 51 bp intron compared with its cDNA.The obtained upstream sequence was 3189 bp,and overlapped 352 bp with cDNA.Analyses indicated that the upstream sequence contained several regulatory...