Chicken-mashed bone was used as experimental material and was hydrolyzed simultaneously by flavourzyme,papain and neutrase.The temperature,pH and the ratio of flavourzyme,papain and neutrase significantly influenced on the content of soluble nitrogen and optimized according to the response surface methodology.The optimal conditions were the temperature of 56℃,pH of 6.2 and the ratio of flavourzyme,papain and neutrase of 3.2:1:1.The analysis of fatty acid from hydrolyzed production showed that the content of...