[Objective] To study the cellular and molecular mechanism of the development of Populus heteromorphic leaves.[Method] SDS-PAGE and 2-DE technique were used to extract and separate the protein in the heteromorphic leaves of adult Populus euphratica,as well as to establish an electrophoretic technique which was suitable for separating the protein in the leaves of P.euphratica.[Result] Significant difference of protein expression was existed in heteromorphic leaves of P.euphratica.SDS-PAGE pattern showed that ...