[Objective]The research aimed to analyze the chemical constituents and anti-tumor activity of petroleum ether extract from Sagina japonica (Sw.) Ohwi.[Method]GC-MS technology was applied to analyze and identify chemical constituents of petroleum ether extract from S.japonica.Three tumor cells were chosen,and MTT and SRB were involved in the process of a screening study on the human leukemia cell lines K562,human cervical cancer cell cine (Hela cell) and human breast carcinoma cell line MCF-7.[Result]25 kind...