[Objective]The research aimed to establish a method to determine the content of icariin in Gukangbao granules (sugar free).[Method]HPLC was adopted with a Kromasil C18 column (200.00 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.5% acetic acid (25:75),the detecting wavelength was 270 nm,the flow rate was 1.0 ml/min,and column temperature was 30 ℃.[Result]The linear range of icariin was within 0.050-0.500 μg(r=0.999 8),and the average recovery was 99.92% with RSD of 0.39%(n=6).[Conclusion]The method wa...