With the methanol-water as the mobile phase,the HPLC external standard method was used to analyze the complex of difenoconazole and thiophanate-methyl by BDS C18 column and UV detection.The results showed that the standard deviations of difenoconazole and thiophanate-methyl were 0.10 and 0.12 respectively with the coefficients of variation as 0.39% and 0.30%;their average recoveries were 99.1%.