The study on the active center of proteinase is crucial to breeding and seeding leaven of Aspergillus oryzae3.042.This study choosed four special proteinase inhibitors such as AEBSF、E-64,Pepstatin A and EDTA,to study their effect to proteinase of seeding leaven which has grown 72 hours,and decided active center of the acid,neutral and alkalious proteinase of Aspergillus oryzae3.042.The result shows that the active center of the acid proteinase is mainly constituted by serine and aspartate,the active center ...