The method of P-dimethylaminobenzaldehyde spectrophotometry was established for determination of L-hydroxyproline in milk powder. After hydrolyzed with acid,L-hydroxyproline was released and oxidized by Chloramine T. L-hydroxyproline oxide reacted with P-dimethylaminobenzaldehyde and formed red compounds under certain temperature,the Absorbance could be detected at 561nm. The liner range of this method was 0.002～3μg/mL,recovery range was 90.6%～94.1% and RSD was less than 1.5%. This method was simple and acc...
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