The study was conducted on the dynamic changes of α-amylase, β-amylase and limit dextrinase during the barley sprouting course by adding different materials (metal ions and GA3). According to experiments, the optimum amount are as follows: Mg2+ 50mg/kg, Ca2+ 50mg/kg, Zn2+ 20mg/kg, K+ 60mg/kg,Na+ 80mg/kg, GA3 0.5mg/kg. Compared to adding ions and GA3 individually, their joint effect of activation on three enzymes is more noticeable.