Objectives: To observe the inhibiting effect of flavone from Oldenlandia diffusa(willd.) Roxb. on HepG-2 cell in vitro. Methods: The effect of cell growth inhibition was observed through the cell shape and apoptosis was assessed by flow cytometry. Results: The flavone from Oldenlandia diffusa(willd.)Roxb. inhibited HepG-2 cell growth and induce apoptosis. Conclusion: The flavone from Oldenlandia diffusa(willd.) Roxb. inhibit HepG-2 cell by induction of apoptosis.