Objective:To extract pulmonary surfactant-associated protein A (SP-A) from derelict whole-lung lavage (WLL) liquid of coal worker patients with pneumoconiosis. Methods: SP-A was purified from the primary production, with its contents and purity determined with chromatogram, and identified with ELISA, and analyzed with JM04 dynamic tension recorder. Results: The average content of SP-A was (1.3692±0.3885) g from each patient, and (92.20±7.00)% in extracted samples. The average surface tension was changed by ...
【更新日期】
2009-05-04
【分类号】
R341
【正文快照】
肺表面活性物质相关蛋白A(Pulmonary Surfactant-associated Protein A,SP-A)是肺表面活性物质(Pulmonary Surfactant,PS)的主要蛋白成分,制备人SP-A对PS基础研究和临床应用具有重要意义[1-4]。2006年10月我们以抗人SP-A单克隆抗体制作亲合层析柱达到了提纯人源性SP-A的目的,报