Using RACE technology,a plant denfensin gene was cloned from Brassica campestris ssp.chinensis genomic DNA.The plant denfensin gene,designated BcDF1.2,contains 532 bp nucleotides with a 96 bp intron sequence (DDBJ accession No.AB302891).BcDF1.2 encodes a polypeptide of 79 amino acids,and showed high degree of homology to other plant defensin genes and antifungal proteins.Phylogenetic analysis revealed the evolutionary conservation of this protein among different plants.Genomic DNA gel blot showed that BcDF1...