Objective: To determine the content of gentiopicrin in different parts of the Gentiana rigescens Franch and evaluate its quality.Methods: The concentration of gentiopicroside was determined by HPLC method;the separation was performed on Agilent ZORBAX SB C18(4.6×150mm,5μm)with methanol-H2O(27∶73)as a mobile phase and the detection wavelength was 274nm at 25℃.Results: The contents of Gentiopicrin in different parts of the Gentiana rigescens Franch were determined.The calibration curve was linear in the range...