Bioleaching test of Zijinshan copper sulfide ore in a shake flask was conducted at different temperatures.Bacteria culture from Zijinshan copper mine bioheap was applied in this test.The highest copper leaching rate of 87.62% in 10 d was achieved at 30 ℃.Microbial community at 15 ℃ and 30 ℃ was analyzed by denaturing gradient gel electrophoresis.The dominant bacteria groups at 15 ℃ and 30 ℃ are Leptospirillum ferriphilum and Acidithiobacillus thiooxidans respectively,together with Acidithiobacillus caldus a...