Adopt different hormone to match ratio the more hurting organization guidance and establishing regeneration on studying to Allium satuvum L.of Majiang,the result showed that:The appropriate medium for different culture stages was: B5+2,4-D 2.0 mg/L;Guide of proper culture medium of indeterminate bud was:B5+6-BA 1.0 mg/L+NAA 0.01 mg/L;optimal for bud multiplication was:B5+6-BA 0.3 mg/L+NAA 0.1 mg/L;better for rooting was:B5+6-BA 0.01 mg/L+NAA 0.1 mg/L.