Objective:to investigate the different effect using different blood needle in neonatal screening for diseases.Method:adopted by 3 different kind of blood needle to 2126(group A), 1786(group B), 1471(group C) with a newborn babies in the blood adoption, then compare their success rate for blood adoption.Result:the difference between A、B、C Group, have statistical significance(P<0.01).Conclusion:the 7th-type injection needles(C group) can significantly improve success rate for blood adoption.