[Objective] The aim was to supply technical support for solving the problem that the sewage was difficult to dispose in north China in winter.[Method] The influences of pH value,temperature and time on the dehydrogenase activity in 8 strains of cold-resistant bacteria isolated,purified and identified from low-temperature biofilm were analyzed by TTC method.[Result] The optimum pH value of the strains S1,S4,S5,S7 and S8 at both optimum temperature and 4 ℃ was 7.5 and that of the other 3 strains at the 2 temp...