With liquiritin as the standard substance,total flavonoids in glycyrrhiza from different areas was determined by ultraviolet spectrophotometry method.The results showed that total flavonoids in glycyrrhiza from Bachu of Xinjiang was the highest(5.62%),and that in glycyrrhiza from Yanchi of Ningxia was the lowest(3.89%).The linear detection range of total flavonoids in glycyrrhiza was 0.524-13.100 μg/ml with average recovery of 100.65% and RSD of 2.24%(n=6).