[Objective] The research aimed to study on the nitrobenzene biodegradation of white rot fungi.[Method] UV was used to detect the activity of manganese peroxidase to obtain the optimal culture environment and GC to confirm the rate of nitrobenzene degradation.[Result] The optimal culture environment of the white rot fungi was as follows: 37 ℃,pH value of 4.5,tween 80 as surfactant.The optimal culture time of white rot fungi are six days and 10 mg/L nitrobenzene can be degraded entirely in seven days.[Conclus...