[Objective] The effect of esterase isoezyme on the classification and identification of Pholiota adipose was studied.[Method] With the vertical flat electrophoresis of polyacrylamide gel,12 Pholiota adipose strains from different regions were compared and analyzed in esterase isozyme character and the genetic differences among strains were analyzed with the cluster analysis software.[Results] There were 23 bands detected with electrophoresis staining,the majority of which showed the difference in isozyme pa...