[Objective] The aim of the study was to enhance the themostability of neutral protease.[Method] The ectoine was supplemented to the neutral protease solutes to study the effect of ectoine on enhancement of thermostability of neutral protease.[Results] It was demonstrated that ectoine could protect neutral protease from thermal inactivation.The neutral protease with 3mmol/L ectoine was incubated at 50 ℃,the residual enzyme activity of neutral protease was increased by 12.7%;the neutral protease with 5mmol/L ...