[Objective] The research aimed to explore the isolation and identification of producing endophytic fungi of berberine from the plant Phellodendron amurense.[Method] The endophytic fungi were isolated from P.amurense and their metabolites were analyzed by improving bismuth potassium iodide reagent and thin layer chromatography.The strains,which may produce berberine,were used in enlarged culture,then the infrared spectrum detection was performed on the extracts produced in the enlarged culture by KBr smearin...