[目的]分析了解乌鳢结节病的病原。[方法]以患病乌鳢为研究材料,通过组织病理学观察和人工感染试验,确定乌鳢结节病的病原性质;通过基本生理生化特性、16 S rRNA基因部分序列和药物敏感性的测定,对乌鳢结节病的病原进行鉴定。[结果]组织病理学观察显示典型结节的中央为干酪样坏死,内含坏死的组织细胞和白细胞,其间有大量呈长杆状或分枝状菌丝;从病鱼的肾脏、肝脏等多个组织及腹水中均分离培养出细菌,用分离菌株作回归感染,证实分离菌株为乌鳢结节病的病原菌;通过对病原菌的基本生理生化特性测定和16 S rRNA基因部分序列分析,初步确定乌鳢结节病的病原为诺卡氏菌属的鰤诺卡氏菌(Nocardia seriolae)。[结论]该研究为乌鳢结节病的进一步研究奠定了基础。
【英文摘要】
[Objective] The aim was to analyze and understand the character and identity of pathogen of Ophiocephalus argus sarcoidosis.[Method] With diseased O.argus as research material,through the observation of histopathology and artificial infection test,the pathogenic character of sarcoidosis of ophiocephals argus;through determination of basic physiological and biochemical characteristics,partial sequence of 16 S rRNA gene and drug sensitivity,the pathogen of O.argus sarcoidosis was identified.[Result] The obser...