[Objective]The research aimed to select the optimum immobilized carrier for nitrile hydratase producing strain.[Method]Nitrile hydratase producing strain Rhodococcus sp.HUST-3 was immobilized by sodium alginate,polyvinyl alcohol,polyacrylamide,chitosan and agar,and then combined.[Result]The results showed that the mechanical strength of sodium alginate-polyvinyl alcohol was high,but batch reaction was only 7 times.Sodium alginate-polyacrylamide had good performance in mechanical strength and phosphate-resis...