[Objective] The research aimed to establish the complete regeneration system of Bergenia crassifolia and lay a foundation for researching its genetic transformation.[Method] With leaves of wild B.crassifolia as explants,its regeneration system cultured in vitro was established preliminarily through screening media and hormone proportions.[Result] The optimum induction medium of B.crassifolia callus was improved MS + NAA 0.50 mg/L + 6-BA 0.50 mg/L + dried hydrolyzed casein 100.00 mg/L + 0.2% PVP.Its differen...