[Objective] The research aimed to seek the suitable deproteinization methods from Momordica charantia L.polysaccharides.[Method] The deproteinization effects of Seveage method,enzyme method,enzyme-Seveage method,trichloroacetic acid method and hydrochloric acid method on M.charantia polysaccharides were compared.[Result] The deproteinization effect of enzyme-Seveage method was the best,with the papain dose of 1.25%(V/V),pH 6.5 at 60 ℃ for 2 h.Then the polysaccharide solution was deproteinized again with Sev...