Aim:To optimize Macroreticular resin in absorption and separation of Neo-gamboge acid from Garcinia hanbyryi Hook f. Methods:The absorption of seven types resin were compared. To optimize the best resins for separating and purification Neo-gamboge acid. Results:AB-8 resin is the best and the static for separating and purification Neo-gamboge acid. 67% of Neo-gamboge acid was eluted from AB-8 resin. Conclusion:AB-8 resin is the most effective one for large scale separating and purification Neo-gamboge acid f...