With the development of plant genetic engineering,the genetic transformation of maize has changed from theoretical research to applied research.Tissue culture is the basal and important part of maize genetic transformation,so it has been paid more attention in maize transgene breeding.Based on the explants of immature embryo,shoot tip and mature embryo of different genotypes maize,the recent research progress,the influencing factors,the problems and the solutions on maize tissue culture and plant regenerati...