Objective: Strength the reliability of detemining the assay for taurine in milk power for infant and young children with pre-column derivatization HPLC. Methods: HPLC method was used, chromatography condition included:The instrument was SHIMADZU-10A with a Kromasil C18 column, and the mobile phase was methanol-0.05mol/L NaAc (35:65) at flow rate of 1.0 ml/min. The UV detection wavelenge was 360 nm. Results: Taurine had a good linearity(γ=0.9996)in the range of 0.00~20.34μg/ ml. The relative recovery was 99....