With the developing of multi-electrode arrays(MEA) technology,it is possible to record the activities of neurons simultaneously.The dissociated rat hippocampal neurons were cultured on a multi-electrode array(MEA) substrate and grew into neuronal networks in vitro.The cross-correlation function method was introduced to analysis the spontaneous firing of spontaneous activity.By analyzing the spike trains on different electrodes detected,the cross-correlation function could identify the relationship of them.T...