HPLC,paper chromatography and Berthelot spectrophotometry were recommended as three methods to assay the content of γ-Aminobutyric acid(GABA) existing in food materials in the present article.It was seen that HPLC,as a widely used method,was sensitive and accurate in GABA analysis.Paper chromatography and Berthelot spectrophotometry,as common methods to assay GABA,were easy for GA-BA analysis from a lot of samples in practice.Moreover,paper chromatography might be used to detect the GABA contained in food m...