Through the experiment of bacteriostasic activity of total flavones in sea buckthorn by the method of agar diffusion,the results showed that strong antimicrobial effects for bacterium. MIC values were:staphylococcus 0.625%,bacillus subtilis 2.5%,E-coli 5%.When the period of reaction time is the same the higher the concentration of the extract the higher the antimicrobial effect. When the concentration is the same reaction period,the higher the antimicrobial effect of the extract.