Response surface methodology(RSM) was employed to optimize culture medium for the laccase production in solid cultivation by Coriolus hirsutus.A fractional factorial design(FFD) was used to screen out the important components for the fermentation substrate.It is inverse proportion of mulberry leaves and bran,oxalic acid ammonium and glucose.The path of steepest ascent was used to approach the optimal response region.The proportion and concentrations of the significant factors were optimized consequently thr...
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