To investigate the effects of GDNF on the proliferation of testis sertoli cells in mouse.Sertoli cells from the testis of the mice aged 5~10 d were isolated and cultured.The effects of GDNF on the proliferation of sertoli cells in vitro were detected by MTT assay.The proliferation of sertoli cells was promoted by GDNF at certain concentration(p<0.05),and significantly promoted by GDNF at 20 μg/L(p<0.01).The change of DNA content was determined with flow cytometry and the cell cycle was analyzed.Results show...