The components and contents of collagen amino acids in some mammal and fishes were studied using HITACHI L-8800 amino acid automatic analyzer,to providing reference for rational development of collagen.The results showed that both of mammal and fishes were rich in 18 kinds of amino acids,in which 8 kinds of essential or conditionally essential amino acids were found.Significant difference in the content of methionine,threonine and hydroxyproline were found between mammal and fishes.