Phospholipid is extracted by the industrial alcohol from pig brain,which is pretreated by acetone for removing the water,cholesterol and fat.Through the orthogonal test,the optimum extraction conditions are determined as:the ratio of solid to liquid of 1:7,extraction time of 1 h,and extraction temperature of 50 ℃.Then the achieved phospholipid is characterized by TLC and infrared spectroscopy.