Objective To establish a method for efficient transfer of 1.3-fold HBV/C genome into HepG2 cell line using adenoviral vector system for studying the replication and antigen expression of HBV.Methods The 1.3-copy overlength genome of HBV genotype C was constructed and cloned into the shuttle vector pAdTrack.After confirmation of the constructed HBV genome by sequencing, the resultant plasmid linearized by digestion with Pme I was transformed into competent E.coli Adeasier-1 cells.The recombinants of pAdEasy-...
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