Objective:To detect the content of diosgenin in stem,leaf,fruit and root of Dioscorea nipponica Makino at different growing period.Methods:RP-HPLC was used to detect the content of diosgenin with a discovery C18 column (250mm×4.6mm×5μm):mohile phase-methanol:water (84:16),flow rate:0.8ml/min, column temperature:room temperature,UV detector:203nm.Results:The linear range of diosgenin was 0.10-1. 0mg/ml,r=0.9999.The average recovery was 97.23%,RSD=0.879%(n=5).The diosgenin content in stem,leaf and fruit of Di...