Objective: To study the quick extraction and determination of icariin in Herba Epimedii.Methods: The orthogonal design and ASE300 system were used to study the extraction method of icariin.The content of icariin was determined by HPLC.The methanol water glacial acetic acid(60∶40∶0.25) was used as a mobile phase. The flow rate was 1.0ml/min,the detection wavelength was 270nm and the column temperature was 41℃.Results: The optimal extraction conditions were: 50% alcohol and extracting one times for twenty min...