[Objective]The aim of this paper was to study the method of seed sterilization and to select the suitable explant and hormone combination for bud proliferation in tissue culture of Flemingia philippinensis Merr.et Rolf.[Method]Different combinations of ethanol,NaClO and HgCl2 were used for seed sterilization,then the comparison between apical bud and stem with axillary buds as explant for subculture was conducted.Finally,the effects of different hormone combinations on bud proliferation were discussed.[Resu...