[Objective] The aim of this study was to optimize the culture condition of wheat mature embryo in vitro.[Method] Two wheat varieties were used to study the factors affecting callus induction of mature embryos from different media and hormone proportions by using orthogonal design.[Result] Among three basic medium of MS,1/2 MS and N6,1/2 MS was the optimal medium for callus induction of mature embryo in wheat.IAA had better effect for Yangfu 2 and KT for Wanmai 41.The optimum medium for quality of callus was...