[Objective] The aim was to study the effects of azotobacter inoculation on the physiological characteristics of nitrogen nutrition in the tissue cultured seedlings of sugarcane.[Method] Two species of endophytic azotobacters B16L and B8R were inoculated into to the tissue cultured seedlings of sugarcane cultivars G16,R16 and R22 so as to study the changes of physiological characteristics of nitrogen nutrition in these tissue cultured seedlings.[Result] Determinations in tillering and jointing stages showed ...