[Objective] The purpose was to study the effect of carbon source on the output of polysaccharide in the liquid-state fermentation of Lentinus edodes hyphae. [Method] With glucose, starch and sucrose as carbon source, the polysaccharide was separated with column chromatography and determined with phenol-sulfate acid method. The orthogonal design was used to study the carbon source of L. edodes fungi fermentation. [Result] The optimum carbon source of producing L. edodes polysaccharide by fermenting with L. e...