Glutenin was extracted from common wheat.Two types of polydextran gel Sephadex G-50 and Sephadex G-100 were used for study of separation and purification of High and Low Molecular Weight Glutenin Subunits by chromatography.The effects were checked by the method of SDS-PAGE.Protein concentration of each fraction was measured by the method of UV spectrophotometer,and the reclaim ratio of protein was calculated.The results showed that protein reclaim ratio of Sephadex G-50 was higher,and it could be used for p...
【更新日期】
2008-03-12
【分类号】
S512.1
【正文快照】
麦谷蛋白分为高分子量谷蛋白亚基(H ighMolecularW eightG lutenin Subunit,HMW-GS)和低分子量谷蛋白亚基(Low MolecularW eight G lu-tenin Subunit,LMW-GS)。二者在决定小麦品质方面的地位都很重要[1~4]。但在实际研究中,由于时间和精力的限制以及研究方法的不同,往往需要将?
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